PBPK Modeling

  • https://www.nuventra.com/resources/blog/difference-pbpk-poppk-modeling/
  • protein is formed with the combination of these acidic, basic (polar) and neutral (non-polar) amino acids. So, if the protein is containing more of basic amino acids it’ll be positively charged and if it’s containing more of acidic amino acids it’ll be negatively charged.
  • Above the isoelectric point, a protein carries a net negative chargebelow it, a net positive charge.
  • protein has its lowest solubility at its isoelectric point. If there is a charge at the protein surface, the protein prefers to interact with water, rather than with other protein molecules. This charge makes it more soluble. Without a net charge, proteinprotein interactions and precipitation are more likely.